Amity School of Applied Sciences, Amity University Gurugram organized seminar on Understanding the nano -protein interface: An investigation with Graphene oxide - Trypsin system on 25th November 2021.
Dr. Sudip Majumder, Assistant Professor, Amity School of Applied Sciences, began with properties of trypsin-GO and their different adsorption isotherms, time dependent SEM, TIRF, CD, fluorescence spectroscopy, SDS-PAGE, protein assay and AFM. He explained about trypsin in detail. He told why nano-protein interactions are chosen and how GO can be characterized by using XRD, RAMAN spectra. SEM of 1:1 Trp-GO at different time intervals, biochemical assay of trypsin using the synthetic substrate was discussed. Pictorial representation of lamda max of p-nitroaniline, graphical representation of biochemical assay of trypsin and GO with BAPNA at different time intervals, circular dichroism spectroscopy of trypsin at different time intervals, fluorescence spectroscopy and atomic force microscopy was also explored. He explained about objectives of the research, understanding the mode of interaction of Trypsin with GO, effects of biochemical and photolytic property of trypsin, the structural and conformational change in trypsin as a result of this interaction and visualization of absorption dynamics.
Students understand different ideas to correlate protein with nanomaterials and produce better research output and would help in advancement of the world of science with ease.
Total 22 students and 3 faculties attended the session.