Faculty

Dr. Krishna Murari Sinha
Associate Professor
Biochemistry, Microbiology, Bioinformatics
Faculty of Science Engineering And Technology

Amity University Haryana, Amity Education Valley, Pachgaon, Manesar, Gurgaon, Haryana 122413

21 YEARS POST PH.D.

CHEMICAL BIOLOGY (BTE2305)
ANIMAL BIOTECHNOLOGY (BTE4111)
MOLECULAR BIOLOGY LAB (BTE2505)
RECOMBINANT DNA TECHNOLOGY(BTE2601/6601)
RECOMBINANT DNA TECHNOLOGY LAB (BTE2605/6606)
STRESS BIOLOGY ( BTH2604/BTH6604)
STRESS RESPONSE SIGNALLING IN MYCOBACTERIUM

Publications:

1. Manikandan, K#., Prasad, D#. Srivastava, A. Singh, N., Dabeer, S., Krishnan, A., Muniyappa, K*, Sinha, K. M*. (2018) The second messenger cyclic di-AMP negatively regulates the expression of Mycobacterium smegmatis recA and attenuates DNA strand exchange through binding to the C-terminal motif of mycobacterial RecA proteins. Molecular Microbiology 109, 600-614. (* corresponding authors)

2. Manikandan, K., Sabareesh, V., Singh, N, Saigal, K., Mechold, U., Sinha, K. M.* (2014) Two-step synthesis and hydrolysis of c-di-AMP in Mycobacterium tuberculosis. PLoS One 9, e86096. (*corresponding author)

3. Kumar, A., Saigal, K., Malhotra, K., Sinha, K. M.* Taneja, B.* (2011) Structural and functional characterization of Rv2966c reveals an RsmD-like methyltransferase from Mycobacterium tuberculosis with role of N-terminal domain in target recognition. J. Biol. Chem. 286, 19652-19661. (* corresponding authors)

4. Sinha, K. M.#, Unciuleac, M.#, Glickman, M. S., Shuman, S. (2009) AdnAB: a new DSB-resecting motor–nuclease from mycobacteria. Genes Dev 23, 1423-1437. (Comment in Genes Dev 23, 1481-1486.)

5. Sinha K.M., Glickman, M. S., Shuman, S. (2009) Mutational analysis of Mycobacterium UvrD1 identifies functional groups required for ATP hydrolysis, DNA unwinding, and chemomechanical coupling. Biochemistry 48, 4019-4030.


6. Sinha, K. M., Stephanou, N. C, Unciuleac, M., Glickman, M. S., Shuman, S. (2008) Domain requirements for DNA unwinding by Mycobacterial UvrD2, an essential DNA helicase. Biochemistry 47, 9355-9364.

7. Sinha, K. M.#, Stephanou, N. C.#, Gao, F., Glickman, M. S., Shuman, S. (2007) Mycobacterial UvrD1 is a Ku-dependent DNA helicase that plays a role in multiple DNA repair events, including double-strand break repair. J. Biol. Chem. 282, 15114-15125.

8. Sinha, K. M., Hines, J. C., Ray, D. S. (2006) Cell cycle dependent localization and properties of a second mitochondrial DNA ligase in Crithidia fasciculata. Eukaryotic Cell. 5, 54-61.

9. Sinha, K. M., Hines, J. C., Downey, N., Ray, D. S. (2004) Mitochondrial DNA ligases from Crithidia fasciculata and Trypanosoma brucei. Proc. Natl. Acad. Sci. USA. 101, 4361-4366. (Comment in Proc. Natl. Acad. Sci. USA. 101, 4333-4334)

10. Sinha, K. M., Ghosh, M., Das, I., Datta, A. K. (1999) Molecular cloning and expression of adenosine kinase from Leishmania donovani: identification of unconventional P-loop motif. Biochem. J. 339, 667-673.
(# represents equal contribution)
MOLECULAR BIOLOGY, BIOCHEMISTRY
TEACHING, RESEARCH
Role of Cyclic di-AMP in ribosome biogenesis, 16S rRNA methyltransferase (RsmD) function and drugresistance in Mycobacterium (SERB, 2017-2020) (Rs. 45.0 lakhs approx)
Identification of cGAMP specific phosphodiesterase from Leishmania donovani and its role in virulence(DBT, Government of India, 2016-2019) (Rs. 60.94 lakhs)
Role of DisA and cyclic-di-AMP in signaling DNA damage & repair and host-pathogen interaction in
mycobacterium (DBT, Government of India, 2013-2016) (Rs. 86.6 lakhs)
Characterization of N6-adenine methyltransferases from mycobacterium and their role in pathogenicity(DST, Government of India, 2012-2015) (Rs. 44.5 lakhs)
Regulation of SOS response in mycobacterium by sigma factors: its role in drug resistance and virulence(DBT, Government of India, 2010-2013) (Rs. 41.62 lakhs)
Thesis Examiner Of Calcutta University
Ad Hoc Reviewer For Plos One, Rsc Advances, Pathogens And Disease
SELECTED FOR INSA VISITING SCIENTIST PROGRAMME, 2019
CSIR FELLOW
FUNDINGS RECEIVED FROM DBT, DST, SERB

Research proposals funded:

1. Role of Cyclic di-AMP in ribosome biogenesis, 16S rRNA methyltransferase (RsmD) function and drug resistance in Mycobacterium (SERB, 2017-2020) (Rs. 35.36 lakhs)
2. Identification of cGAMP specific phosphodiesterase from Leishmania donovani and its role in virulence (DBT, Government of India, 2016-2019) (Rs. 60.94 lakhs)
3. Role of DisA and cyclic-di-AMP in signaling DNA damage & repair and host-pathogen interaction in mycobacterium (DBT, Government of India, 2013-2016) (Rs. 86.6 lakhs)
4. Characterization of N6-adenine methyltransferases from mycobacterium and their role in pathogenicity (DST, Government of India, 2012-2015) (Rs. 44.5 lakhs)
5. Regulation of SOS response in mycobacterium by sigma factors: its role in drug resistance and virulence (DBT, Government of India, 2010-2013) (Rs. 41.62 lakhs)
Faculty of Science Engineering And Technology
Amity University Haryana, Amity Education Valley, Pachgaon, Manesar, Gurgaon, Haryana 122413