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Central Instrument Research Facility Download CIRF Booklet

Central Instrument Research Facility, AUH

The central instrument research facility (CIRF) is housed at Amity University Haryana, Gurugram provides a central facility of latest and advanced analytical instrument for research and for promoting interdisciplinary research. Instruments are manned by qualified personnel. This facility is available for Amitians and other academic research institutions. industries and organizations.

Bookings

External User Amity User

Imaging facility

Features
  • Largest field of view (25 mm) in its field, when combined with the justified Ti 2-E inverted microscope, the imaging area of A1 R HD 25 is nearly twice the conventional FOV of 18 mm.
  • High definition imaging up to 1k x 1K, 1024 x 1024 pixels enables acquisition of high resolution, high quality images at lower magnification, enabling compatibility with a wide range of samples.
  • High speed imaging capability up to 720 fps, in combination with a large field of view, dramatjustiically increases imaging throughput. The scanning method reduces the exposure time of the sample to excitation light, minimizing photo toxicity and photo bleaching
  • Capture large scale overview images as well as high magnification images with the same instrument. The 25 mm FOV of the A1R HD 25 is effective for observation of large samples, while its 1k x 1k high definition is ideal for the observation of minute structures.
  • High sensitive detector options for various types of florescence labels.
Imaging facility

Nikon confocal microscope (Model:-A1R HD 25)

Confocal Microscopy can be useful for

3D imaging, Co localization, FLIP*, Fluorescence, FRAP*, FRET,* Microscopy, Photo activation, Time lapse*.

*Some applications are limited to certain cell/tissue types due to unavailability of a live cell imager at the moment. Kindly consult with the faculty in charge for further information.

Samples

Tissue culture dishes, slide chambers, microplates, glass slides etc. can be used with ADHERENT/FIXED samples. Operating Scientist/Operator must be consulted regarding the thickness/material of slides/dishes required for sample preparation before booking the facility. Control samples without any fluorophore and with fluorophore are required.

Charges*
Users types Confocal microscope
Amity University Rs 1000/hour
Academic & Research institution Rs 2000/hour
Private industries/other laboratories Rs 3000/hour

*Inclusive Charge – 18% GST

Features
A1 R (Nikon Microscope)

A1 R (Nikon Microscope)

  • A fluorescence microscope uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study specimens.
  • A sample is illuminated with light of a wavelength which excites fluorescence in the sample.
  • Typical components of a fluorescence microscope are a light source, excitation filter, the dichroic mirror (or dichroic beamsplitter), and emission filter.
  • Fluorescence microscopes can be used for many different applications including localization of specific proteins, determining the shape of organs, cells, and intracellular structures, studying protein interactions or conformation, examining ion concentration and observing live cells.
  • Look for ease of use, ergonomic features, multiple modes, high resolution and the possibility to upgrade.
Microscopy can be useful for
  • Imaging structural components of small specimens, such as cells
  • Conducting viability studies on cell populations (are they alive or dead?)
  • Imaging the genetic material within a cell (DNA and RNA)
  • Viewing specific cells within a larger population with techniques such as FISH
Samples

Tissue culture dishes, slide chambers, microplates, glass slides etc. can be used with ADHERENT/FIXED samples. Operating Scientist/Operator must be consulted regarding the thickness/material of slides/dishes required for sample preparation before booking the facility. Control samples without any fluorophore and with fluorophore are required.

Charges*
Users types Fluorescence Microscope
Amity University Rs 100/hour
Academic & Research institution Rs 500/hour
Private industries/other laboratories Rs 800/hour

Lipidomics facility

Amity Lipidomics Research Facility Click Here

Spectroscopy Facility

Features
  • Measuring wave length range of the instrument is 190-1100 nm.
  • Manual polarizer holder accessory.
  • Multi cell holder and peltier system for heating upto 100 °C.
  • Horizontal slit orientation for increased viewing.
  • Light source: Xenon pulse lamp
  • Wavelength accuracy: 0.5 – 0.07 nm.
  • Wavelength reproducibility: 0.1 nm
  • 2 bandpass filters in the range of 250–395 nm and 335–620 nm
  • WinFLR Software
Cary Eclipse Spectrofluorometer (Agilent)

Cary Eclipse Spectrofluorometer (Agilent)

Charges*
Users types Spectrofluorometer
Amity University Rs 500/hour
Academic & Research institution Rs 600/hour
Private industries /other laboratories Rs 1000/hour

*Inclusive Charge – 18% GST

Fluorescence Spectrophotometer can be used for
  • Fluorescence, phosphorescence, chemiluminescence and bioluminescence studies
Samples
  • The cuvette should contain the liquid or solution in the spectrofluorometer sample area.
Features
Cary 100 UV-Visible spectrophotometer (Agilent)

Cary 100 UV-Visible spectrophotometer (Agilent)

  • Double beam UV/Vis spectrophotometer, wave length range is 190 to 1100 nm with R928 PMT detector
  • Versatile set of accessories   including peltier system, multicell liquid sample holders (cuvette as small as 8 µl)
  • Wavelength accuracy ± 0.02 nm; UV Vis limiting resolution 0.189 nm.
  • WinUV software  – modular design provides a wide range of applications via a simple interface
UV-Visible spectrophotometer can be used for
  • Nanomaterial characterization, detection of organic materials in water, food and agriculture
  • DNA and protein quantification and measuring small volumes
  • Monitoring kinetics of reactions that occur at sub-second rates
  • Study of denaturation of biomolecules at elevated temperature
Samples

UV-Visible spectroscopy is used primarily to measure absorbance of liquids or solutions. In this technique, the cuvette should contain the liquid or solution in the spectrophotometer sample area.

Charges*
Users types Spectrofluorometer
Amity University Rs 300/hour
Academic & Research institution Rs 400/hour
Private industries /other laboratories Rs 750/hour

*Inclusive Charge – 18% GST

Features
  • Spectral range 7000–350 cm-1 with KBr optics.
  • Wavenumber reproducibility 0.005 cm-1.
  • Single Reflection Diamond ATR for solid or liquid sample. (360° swivel press available)
  • Resolution Pro software and Spectral Libraries of 10,000 compounds including pharma, polymer etc
FTIR can be used for:

The Agilent Cary 630 FTIR spectrometer is versatile, innovative, and intuitive, providing superior quantitative and qualitative information for routine analysis of solids, liquids, and gases.

Cary 630 FTIR Spectrometer (Agilent)

Cary 630 FTIR Spectrometer (Agilent)

Samples

Minimum 5 mg dried and finely grounded samples are required for analysis.

Only powdered and viscous liquid samples (compatible with KBr) will be entertained. The scanning range will be is 4000-400 cm-1.

Charges*
Users types Spectrofluorometer
Amity University Rs 300/sample
Academic & Research institution Rs 1000/sample
Private industries /other laboratories Rs 2000/sample

*Inclusive Charge – 18% GST

Cell Analysis Facility

The BD FACSLyric™ is a high performance flow cytometer designed to support both routine clinical analysis and clinical research. With an integrated solution that combine instrument, software reagents and services, bd facslyric™ provides. clinicians and scientists with accurate, reliable and repeatable result -test to test, instrument to instrument and site to site, regardless of the assay complexity or operator experience level.

BD FACSLyric™ Flow Cytometer

BD FACSLyric™ Flow Cytometer

  • Solid-state laser specifications
  • Blue laser: 488 nm, 20 mw
  • Red laser: 640 nm, 40 mw
  • Optics: excitation lasers upto 10 color with below configuration
  • 6-color: 2-laser (blue, red) (4-2)
  • Detection channels
  • Forward scatter (fsc), side scatter (ssc) and up to 10 fluorescence

A flexible, high-performance instrument in a compact footprint. The system is available in 4, 6, 8, 10 or 12 colors and equipped with a blue, red and violet laser depending on the configuration.

  • Absorbance /excitation 488 nm (blue), 640 nm (red), 405 (violet)
  • The fluidics design enables a large selection of sample input devices. For manual acquisition, choose from 12 x 75-mm tubes, microcentrifuge tubes (~500-μL) or large (up to 50-mL) conical tubes for continuous sample acquisition. For automated acquisition, the optional BD FACS™ Universal Loader provides walkaway operation with samples loaded in either microtiter plates or 12 x 75- mm tube racks.
  • Runs at up to 35,000 events per second
  • Can achieve sample carryover =0.05%
  • No limit on events acquired
Charges*
Users types Spectrofluorometer
Amity University Rs 500/ hour
Academic & Research institution Rs 750/ hour
Private industries /other laboratories Rs 1000/ hour

*Inclusive Charge – 18% GST

FACS can be used for
  • Cellular uptake studies
  • Cancer Studies
  • Detection of apoptosis with Annexin V
  • Cell Cycle analysis

Analytical facility

UPLC H-Class (Waters)
  • The Ultra Performance Liquid Chromatography (UPLC) is used for separation of individual compounds in a mixture.
  • It is equipped with PDA detector and quaternary solvent system . It is one of the widely used techniques in the fields like: clinical research, biochemical research and Industrial quality control, etc.
  • The principle involved in UPLC testing is the separation of compounds in a mixture. Segregation of compounds is due to their relative differences in their retention time or RT. For example, all the monoamines like dopamine, epinephrine, and serotonin can be separated and estimated in a single run.
BD FACSLyric™ Flow Cytometer

BD FACSLyric™ Flow Cytometer

UPLC H- Class can be used for
  • Determination of Pesticides in Groundwater
  • Improved Resolving Power in Peptide Maps
  • Rapid Dose Formulation Analysis 
  • Analysis of Traditional Chinese Medicines (TCM)
  • Identification of Metabolites
  • In Manufacturing / Quality Assurance (QA) / Quality Control (QC)
Samples
  • All the chemicals and reagents used for the process should be of UPLC grade for efficient and smooth analysis.
  • The sample should be dissolved in the eluent to be used and filtered through 0.45μm disposable filter before injection into UPLC system (5 to 10μL).
  • The injection volume of standards and that of actual sample should be the same.
  • Analysis of Traditional Chinese Medicines (TCM)
  • The recordings (preferably in the form of quantitative peaks) are compared with those of standard, and the individual compounds are identified.
Equipment Experiment Amity University Academic& Research Intitutions Private Industries/ other Laboratories
UHPLC** Mass spectrometry 1. ESI-MS based analysis Nominal mass/MW. Determination* Rs.400 per sample Rs.800 per sample Rs.1500 per sample
2. MS/MS analysis* Rs. 300(per Precursor ion) Rs.600 (per Precursor ion) Rs.1500 (per Precursor ion)
3. UPLC-MS analysis (Qualitative) Rs. 1000 (per Precursor ion) Rs.2000 (per Precursor ion) Rs.4000 (per Precursor ion)
4. UPLC-MS/MS Analysis (Qualitative) Rs.2500/sample (5 peaks, Rs 200 per additional peak) Rs.5000/ sample (5 peaks, Rs 300 per additional peak) Rs.10000/ sample (5 peaks, Rs 400 per additional peak)
5. UPLC-MS/MS for Targeted analysis of Lipids/Small molecules Rs 1000/ MRM/analytical lipid unit Rs 2500/ MRM/analytical lipid unit Rs 4000/ MRM/analytical lipid unit
6. UPLC-MS/MS for Targeted analysis of Lipids/Small molecules including data analysis by MultiQuant Software Rs 1500/ MRM/analytical lipid unit Rs 4000/ MRM/analytical lipid unit Rs 5000/ MRM/analytical lipid unit
7. UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules Rs.1500/sample Rs.2500/ sample Rs.5000/ sample
8. UPLC-MS/MS for untargeted analysis of Lipids/Small molecules including data analysis by Lipid view Software Rs.2500/ sample Rs.5,000/ sample Rs.10,000/ sample
9. UPLC-PDA (Qualitative Analysis)* Rs.1000/ day Rs.1,500/ sample Rs.4,000/ sample
10. UPLC-PDA (Quantitative Analysis)* Rs.1000/ day Rs.2,500/ sample Rs.4,000/ sample
User Charges List*

*Experiment no. 1 and 2. Would be done only for pure compound.
**Instrument use only for AUH faculty charge Rs. 500/ per day (all reagent, column, guard column, solvents, standards provide by user).
*Inclusive Charge – 18% GST

Beckman Coulter Optima XPN-100 Ultracentrifuge
Beckman Coulter Optima XPN-100 Ultracentrifuge
  • Beckman Coulter’s Optima XPN-100 Ultracentrifuge is a high performance centrifuge optimized for spinning its rotor at speeds approaching 100,000 rpm or 802,000 x g.
  • Maximum speed 100,000 rpm with speed control of +- 2 rpm of set speed (above 1,000 rpm).
  • Large touch screen display, energy efficient and real time run graphing.
  • Optima XPN boasts additional enhancement that will simplify use, optimize control and security and increase productivity.
Ultra centrifuge can be used
  • To separate components of a sample through the use of relative centrifugal force.
  • It has a wide range of applications ranging from separating cell organelles to measuring conformational changes in proteins
Charges*
Users types Ultracentrifuge
Amity University Rs 800/ day or 200/ hour
Academic & Research institution Rs 1500/ day or 500/ hour
Private industries /other laboratories Rs 3000/ day or 1000/ hour

*Inclusive Charge – 18% GST

Avanti JE Biosafe cold centrifuge (Beckman Coulter)
  • Speed range is 300 to 21,000 rpm, refrigerated floor and temperature range is -10 oC to 40 o C
  • The Avanti J-E system generates maximum speeds up to 21,000 rpm (53,300 x g) and offers the capacity of processing from up-to 4 liters in under 10 minutes.
  • It achieves a high degree of separation in a size compatible with your operations.
  • The Avanti J-E's high torque switched reluctance (*SR) direct drive technology achieves faster acceleration and deceleration with gentle precision that delivers the fastest separations in the shortest run times, unattainable by standard high speed centrifuge systems. Shorter run times mean more discovery time for you.
  • For clinical research applications, conical tubes and bottles for swinging bucket rotors spin volumes of 15, 50, 250, and 500mL for effective blood component isolation with a variety of gradient kits.
Cold centrifuge can be used for

Beside routine centrifuge applications such as cell pelleting from large volumes of cultures, it can be used for separation of macromolecules/ ligand binding kinetic studies, separation of various lipoprotein fractions from plasma and deprotonisation of physiological fluids for amino acid analysis.

Charges*
Users types Cold centrifuge
Amity University Rs 200/ hour
Academic & Research institution Rs 500/ hour
Private industries /other laboratories Rs 800/ hour

*Inclusive Charge – 18% GST

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Team

Director

Director, of Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, AUH

Prof. Rajendra Prasad

Prof. Rajendra Prasad
Director,
AIB/AIISH, AUH
Email: rprasad@ggn.amity.edu

Eminent Scientist, Prof. Rajendra Prasad, is the Director of Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, AUH. Dr Prasad is a former Professor & Dean of School of Life Sciences at Jawaharlal Nehru University (JNU), New Delhi.

He is a leading molecular mycologist and has done trend setting research in the area of antifungal clinical drug resistance

CIRF Faculties

Dr. Kaustav Bandyopadhyay
Assistant professor ( AUH)

Mrs. Kanchan Pandey
CIRF Manager (AUH)

Mr. Shiv nandan
Technical officer (AUH)

Mr. Saurabh Sharma
Technical officer (AUH)

Mr. S.M.Haseeb Faheem
Technical officer (AUH)

CIRF Members

Dr. Atanu Banerjee
Assistant professor ( AUH)

Dr. Gargi Bagchi
Associate professor ( AUH)

Dr. Amit Pandey
Assistant professor ( AUH)

Dr. Anurag Sharma
Assistant professor ( AUH)

Dr. Krishna Murari Sinha
Associate professor ( AUH)

Dr. Nitai Debnath
Assistant professor ( AUH)

Contact Us

CIRF Manager
AIB/AIISH, A- Block, 2nd floor
Amity University Haryana, Panchgaon, Manesar
Haryana-122413, India
Tel: 0124-2337016
Email ID: auhcirf@gmail.com